|
KMID : 1191520060110010055
|
|
Journal of Korean Traditional Oncology
2006 Volume.11 No. 1 p.55 ~ p.63
|
|
|
Angiogenic inhibitory effect of Zingiberis Rhizoma
|
|
Nam Sang-Choon
Kang Hee
Shim Bum-Sang
Kim Sung-Hoon
Choi Seung-Hoon
Ahn Kyoo-Seok
|
|
|
Abstract
|
|
|
|
This study was conducted to investigate angiogenic inhibitory effect of Zingiberis Rhizoma methanol extract using ECV-304 cells and HT1080 fibrosarcoma cells. The viability of ECV-304 was 30% at 50㎕/㎖ of Zingiberis extract and that of HT1080 was 30% at 100㎍/㎖. Using the BrdU incorporation assay, Zingiberis inhibited the DNA synthesis of ECV-304 and HT1080 by 70% and 50 % at 200㎍/㎖. In tube formation assay, at 10㎍/㎖ of Zingiberis, tube network began to degrade and at higher doses, it was completely destroyed. Zymography demonstrated that Zingiberis extract decreased MMP-9 at 10㎍/㎖ and higher doses remarkably inhibited the expression of MMP-9. These data indicate that Zingiberis Rhizoma has angiogenic inhibitory effects and shows the possibility of future anti-metastatic drug.
|
|
|
KEYWORD
|
|
|
Zingiberis Rhizoma , angiogenesis , ECV304 , HT1080 ,
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|